The DeNovix® DS-11 spectrophotometer is a compact, stand-alone instrument with clear advantages over leading
competitors. The table below highlights some of the key features and advantages of the DS-11 versus the
*An erroneous measurement can occur if an insufficient sample volume is pipetted for the
microvolume measurement. In addition, many microvolume spectrophotometers such as
the NanoDrop™ 2000 can exhibit a phenomenon referred to as “unconditioning” where the
surface loses its hydrophobic property and droplets spread out instead of beading up. The
NanoDrop™ 2000 does not alert the user to a broken column and allows erroneous
measurements to go undetected. The DS-11 SmartPath Technology detects if a sample
does not fully bridge the path gap and automatically compensates for the issue in in real
The next generation DeNovix DS-11 introduces new features to the microvolume spectrophotometer market,
providing more confidence for a broader range of sample types and sample concentrations.
The DeNovix DS-11 FX Series and the NanoDrop™ One are used for rapid quantification of nucleic acids and proteins.
DeNovix DS-11 FX Series Spectrophotometer / Fluorometers offer best-in-class limits of detection, the broadest
dynamic range, intuitive ease-of-use, and powerful networking features that are superior to the NanoDrop™ One. This
note presents a comparison of features and side-by-side instrument performance.
The DeNovix team includes one of the founders of NanoDrop Technologies Inc. as well as core team members that
were involved with that company. The DeNovix DS-11, introduced to the market in 2013, was the first Android
controlled, touchscreen and network-connected UV-Vis spectrophotometer in the world. In collaboration with the
Life Science community, DeNovix has expanded the instrument’s features and functionality. In 2015, a highly sensitive
fluorometer was integrated into the system creating the DS-11 FX Series. The patented (US9442009) DS-11 FX+ features
best-in-class limits of detection for microvolume absorbance (0.75 - 37500 ng/μL dsDNA) and fluorescence
quantification (0.5 pg/μL dsDNA). Labs select the integrated model that fits their current and future research needs
by combining microvolume UV-Vis, cuvette UV-Vis and fluorescence detection in one unit. DeNovix includes an
industry leading three year factory warranty on all instruments.
DeNovix spectrophotometers employ proprietary algorithms and hardware that
overcome previous limitations of microvolume instruments. The DS-11 begins analysis at a
pathlength of 0.5 mm and compresses the sample during the measurement until the
optimum pathlength is reached. The DS-11 FX Series does not stretch the droplet. This
prevents an unbridged condition (broken column) that can lead to inaccurate results.
On systems that rely on stretching samples, a broken column may occur if insufficient
sample volume is pipetted for a microvolume measurement, if samples have low surface
tension (common for protein solutions), or if the measurement surface loses its
hydrophobic property. With BridgeTesting, DeNovix detects if a sample is not bridging the
optical surfaces and automatically compensates to provide a correct measurement in real time. Conversely, the
NanoDrop™ One stretches the sample column to 1.0 mm and only alerts the user to a sample column anomaly and
does not report a measurement result.
DeNovix manufactures three ranges of highly sensitive and selective dsDNA assays. These assays, when combined
with the DS-11 FX Series, enable quantification of dsDNA to 0.5 pg/μL, which is 1000x greater sensitivity than
microvolume spectrophotometers. Each assay is a simple two-point standard, mix-and-measure kit. The assays allow
accurate quantification of dsDNA in the presence of RNA, degraded DNA and other contaminants.
A 40 mg/ml solution of dsDNA was gravimetrically prepared using biotechnology grade fish sperm DNA sodium salt
(Amresco cat #1B1509-256) and HPLC grade water (Ricca cat #9153-1). A series of dilutions from 12500 ng/μl to 0.75
ng/μL was then prepared in HPLC grade water.
Reference concentrations for the dilutions were determined using an Agilent 8453 (Agilent, Santa Clara CA) in a 1
mm quartz cuvette (Starna, cat #1-Q-1). The reference value for DNA solutions with absorbance values outside of the
Agilent's upper range of 2.0 A (equivalent to 1000 ng/μL dsDNA) were determined by gravimetrically diluting these
samples to fall within the linear range of the reference spectrophotometer. Measurements were made using the
dsDNA app for each instrument.
Due to the challenges of creating high concentration dsDNA samples (600-750 A), performance was evaluated using
nicotinic acid and the Custom Formula Methods app on the DS-11. An 18.5 mg/mL solution of nicotinic acid was
prepared gravimetrically using 99.5% Nicotinic Acid (Sigma cat# 72309-100) and 0.1 N HCl (Fisher cat# SA50-1) (Ricca
cat# 9153-1). A series of dilutions was prepared from 18.5 mg/mL to 13.5 mg/mL.
The applicable measurement app was launched on each instrument and a microvolume mode Blank measurement
was made using 1 μL of HPLC grade water. Five sample measurements were then made for each concentration.
Fresh 1 μL aliquots were used for each replicate measurement. The sample solution was removed after each
measurement by wiping the upper and lower sample surfaces with a clean, dry laboratory wipe.
The Fluoro dsDNA app of a DS-11 FX+ Spectrophotometer / Fluorometer and DeNovix dsDNA quantification assays
were used in the quantification of dsDNA. Each assay was prepared as described in the manufacturer’s protocol.
Samples were mixed and incubated at room temperature for 5 minutes. Three replicate measurements were taken
for each sample. DeNovix assays were measured on the DeNovix fluorometer.
DeNovix Broad Range and High Sensitivity Assays: A series of dilutions of calf thymus DNA was prepared in TE buffer.
Working solution (190 μL) was added to a thin-walled, clear UV-transparent 0.5 mL PCR tube (DeNovix cat# TUBEPCR-
0.5-500). dsDNA (10 μL) was added to each tube in the standard range, and volume was adjusted for total mass
in the extended range.
Ultra High Sensitivity Assay: Working solution (200 μL) was added to a thin-walled, clear UV-transparent 0.5 mL PCR
tube. dsDNA (10 μL) was added to each tube.
UHS = DeNovix dsDNA Ultra High Sensitivity Assay (0.5 pg/μL- 300 pg/μL)
HS = DeNovix dsDNA High Sensitivity Assay (5.0 pg/μL - 250 ng/μL)
BR = DeNovix dsDNA Broad Range Assay (0.1 ng/μL - 4000 ng/μL)
Abs = Absorbance
Although the nicotinic acid samples were not measured using the
dsDNA app, the ng/μL equivalent values for samples with the high
absorbance values measured are presented as a point of reference.
The DeNovix DS-11 Spectrophotometer delivers greater accuracy and sensitivity than the Thermo Fisher Scientific
NanoDrop™ One. The DeNovix instrument also offers a broader dynamic range, greater ease of use and provides
researchers with superior flexibility and functionality. The DS-11 FX Series combines microvolume absorbance
and fluorescence measurements on one instrument to ensure the user has the tools to measure both routine and
difficult samples. With the DS-11 FX Series, labs are equipped for today’s workflows as well as for the challenges of
future assays. The DS-11 FX Series and DeNovix assays combined provide superior sensitivity and a much broader
dynamic range for dsDNA quantification (0.5 pg/μL to 37500 ng/μL) than the NanoDrop™ One.
2017 Reviewers’ Choice Life Science Product of the Year: The DeNovix DS-11 FX Spectrophotometer / Fluorometer. The DS-11 FX Series combines fluorescence, microvolume absorbance, and optional cuvette absorbance methods in a single, compact instrument.
Rapidly quantify nucleic acid and protein samples over the broadest dynamic range of any microvolume spectrophotometer available.
Fluorescence Freedom! DeNovix fluorometers give scientists the flexibility to choose any fluorescent assay and come pre-configured with a wide range of commercially available quantification assays. Four fluorescent channels encompass a wide fluorophore excitation (375-662 nm) and emission 435-740 nm) range and our EasyApps® software make it simple to set up, save and run new assays.
Microvolume UV-Vis Mode: Always Calibrated, Always Accurate. Be confident in 1µL UV-Vis results, even for difficult to measure samples like protein, thanks to SmartPath® Technology. Our patented design controls the pathlength in real-time while compressing the sample during analysis. SmartPath® ensures the DS-11 FX is always calibrated – no drift and no service downtime.
Easy to learn. Fast to use. DS-11 FX Series instruments include intuitive, pre-installed DeNovix EasyApps® and a breakthrough Android™ touchscreen interface.
Every instrument is ready for absorbance and fluorescence measurements right out of the box – no PC set-up or software installation.
Connect to Your Results. Built-in Wi-Fi, Ethernet, and USB allow easy export of the data you want in the format you need.
Email a .csv or color .jpg of your results…save data to LIMS or network drives…print results or graphs to network and label printers…and
Till date, 123 Scientists have reviewed DS-11 FX+ Spectrophotometer / Fluorometer & Given Average Rating: 4.9
The original innovators of 1µL UV-Vis Spectrophotometers set a new standard - The DeNovix DS-11.
The DeNovix DS-11 takes UV-Vis spectrophotometry to the next level. Its compact, stand-alone design incorporates unique solutions that address all of the common sources of error and frustration found in first generation microvolume spectrophotometers.
The DS-11 is technologically superior and delivered in an interface so intuitive; you already know how to use it.
Confidence Built-In Combining two new breakthrough technologies, SmartPath® Technology and BridgeTesting® the DS-11 is Always Accurate, Always Precise and Always Calibrated. The proprietary algorithms and optomechanical components ensure sample columns are intact and
that the path length is always calibrated. These are often undetected sources of error for other microvolume spectrophotometers.
Simple, Fast and Connected: How do you need your data? Saved to your network drive? Printed on a table? Need the UV/Vis spectra overlaid? Need a label for the tube? No problem. Our stunning custom Android™ operating system with EasyApps® software gives you WI-FI, Ethernet, USB and all the options you need to get your data just where you need it. Pre-installed software with dedicated apps for each application means it’s
ready to run right out of the box and is easy to navigate, fast to operate software gets you from sample data in seconds.
Till date, 68 Scientists have reviewed DS-11 UV-Vis Spectrophotometer & Given Average Rating: 4.8
The highly sensitive QFX Fluorometer is a powerful solution for fluorometric applications. Quantify nucleic acids and proteins using four fluorescent channels for
running all the common quantification assays or easily create custom assays to take advantage of new fluorophores.
Fluorescence Freedom! DeNovix fluorometers give scientists the flexibility to choose any fluorescent assay, not just a specific one designated by a manufacturer. Fluorometer apps are pre-configured for common commercially available assays measured in standard 0.5 mL thin-wall PCR tubes.
Four fluorescent channels encompass a wide fluorophore excitation (375-662 nm) and emission (435-740 nm) range.
Easy to learn. Fast to use. The QFX includes pre-installed DeNovix EasyApps® and is ready for fluorescence measurements right out of the box. Utilize intuitively designed apps for nucleic
acid and protein quantification or easily create custom fluorometric methods for your own applications.
Connect to Your Results. Built-in Wi-Fi, Ethernet, and USB allow easy export of data you want in the format you need.
Email your results…save data to LIMS, USB or network drives…print results to network and label printers…and more!
The DeNovix DS-C is a simple-to-use UV-Vis cuvette spectrophotometer with outstanding dynamic range, sensitivity, and flexibility. Rapidly quantify biomolecules using any cuvette with an 8.5 mm z-height. The instrument
includes preinstalled EasyApps for nucleic acids, proteins, colorimetric assays, OD600, Kinetics, Custom Methods and more.
The DS-C features the most powerful and intuitive software in its class. Pre-installed DeNovix EasyApps® and a breakthrough
Android™ touchscreen interface ensure error-free operation and sample results under 2 seconds. Every instrument is ready right out
of the box and backed by an industry-leading three-year warranty.
Add a DeNovix USB FX Module to enable powerful fluorescence quantification using the DS-C. DeNovix Fluorescence EasyApps are
preloaded on the instrument and instantly become enabled when the USB model is connected. Use fluorescent assays for dsDNA, RNA and
protein quantification from DeNovix or several other manufacturers. Four fluorescent channels encompass a wide fluorophore excitation
(361-662 nm) and emission (435-740 nm) range. Learn more about the FX Fluorometer Module.
Built-in Wi-Fi, Ethernet, and USB allow easy export of the data you want in the format you need.
32 GB of memory allows storage of millions of results!
DS-C Spectrophotometers are available in your choice of four DeNovix colors.
DeNovix dsDNA Fluorescence Quantification Assays: From single cell to Maxi Prep: An assay choice for all applications.
The DeNovix dsDNA Fluorescence Quantification Assays include three highly-specific, highly-sensitive kits for dsDNA quantification.
Selectively and accurately measure dsDNA in samples at single cell levels even if samples include contaminants such as RNA, ssDNA or protein.
DeNovix dsDNA assays cover three overlapping ranges of sensitivity. Each kit includes standards for easy set up of a two-point standard curve.
DeNovix assays have a wider dynamic range than Qubit® dsDNA assays and are quick and easy to run. Our breakthrough dsDNA Ultra High Sensitivity assay
allows measurement of samples as low as 0.5 pg/µL (original sample concentration), 20X lower than the core range of the Qubit® dsDNA HS assay.
Assays are available in 1000 assays/kit size, or 50 assays/kit size, or 250 assays/kit evaluation size.