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Double Beam UV Visible Spectrophotometer available as either a standalone instrument of a PC-control

Double Beam Spectrophotometer - (UV 3200)

The two detectors measure sample and reference respectively and simultaneously for optimizing measurement accuracy. They provide excellent performance for measurements in the range of 190 to 1100nm. They are suitable for pharmaceutical, biochemical and clinical lab applications as well as routine applications such as quantitative analysis, kinetics, wavelength scan, multiple components  and DNA/protein.

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Standard Features of Double Beam Spectrophotometer - (UV 3200)
  • PC Windows application software make these instruments versatile.
  • Variable slits (bandwidths)
  • For stand-alone instruments, all software methods are included as built-in standard, this eliminates the need of software.
  • Online software upgrade via internet helps to keep it updated.
  • Data Download-to-PC software expands the data storage to unlimited.
Typical Specification

 

Specifications

Double Beam Optical System with Automatic 8-Cell Changer - Model 3200

Wavelength Range

190nm to 1100nm

Working Mode

Stand alone and PC controlled with window based application software UV/\/IS Analyst

Spectral Band Width

Variable 0.5, 1, 2, 4 nm

Monochromator

Double beam hollow graphic matings 1200 lines/mm

Wavelength Display

0.1nm

Wavelength Setting

0.1nm

Wavelength Accuracy

± 0.1 nm @656.1nm D2

 

± 0.3nm (190 to 1100nm)

Wavelength Repeatability

<0.1nm

Stray Light

0.02°%@ 340nm for NaN02

 

0.25% @ 198nm for Kcl

Optional Accessory

Specular Reflection Accessory, Integrating Sphere 60mm / 100 mm Automatic 8 Cell changer, Rectangular cell holder for variable path length, Large sample compartment having stable and reproducible mounting of accessories for easy use of different cells., Peltier Solid Sample holder etc.

Photometric Range (Approx.)

0-400%T  Absorbance  -4  to 4

Photometric Accuracy

± 0.002 Abs(0.5)

 

± 0.004 Abs(1.0)

 

± 0.006 Abs(2.0)

Photometric Reproducibility

0.001 Abs(0.5 Abs)

 

0.001 Abs(1.0 Abs)

 

0.003 Abs(2.0 Abs)

Baseline Stability

<  0.0002 Abs/H @ 500 nm

Baseline Flatness

± 0.0005 Abs

Noise Level

0.000016 Abs RMS @ 500 nm

Scan Speed

1-6000nm per Minute

Light Source

Tungsten and Deuterium Lamp, pre-aligned

Output

USB Port and Parallel Port (Printer)

DNA/Protein Measurement

Included

Dimension (W x D x H)

600 x 450 x 200 nm

Weight

22kg

Memory

USB Memory Devices (standard accessory)

Wavelength Slew Rate

6500 nm/min.

Lamp Interchange Wavelength

Automatic interchange linked to wavelength. The interchange wavelength can be set freely.

Detector

Silicon Photodiode (Dual)

The PC Models come standard with windows based application sofMare UV/Vis Analyst

FAQs

1. What does a UV spectrophotometer measure?

UV-Vis Spectroscopy (or Spectrophotometry) is a quantitative technique used to measure how much a chemical substance absorbs light. This is done by measuring the intensity of light that passes through optical components with respect to the intensity of light through a reference sample or blank.

2. What are the main components of a UV VIS spectrophotometer?

There are four basic components to a simple single beam UV/Vis spectrophotometer; a light source, a monochromator, a sample, and a detector.

3. What is the difference between UV and visible spectrophotometry?

Molecules having non-bonding electrons can absorb the energy in the form of UV or visible light to excite these electrons to higher molecular orbitals. ... Ultraviolet-Visible Spectroscopy is absorption spectroscopy in the UV and visible portion of the electromagnetic spectrum.

4. Is infrared radiation dangerous?

In general, no -- at least from naturally occurring physical processes. Any form of radiation -- including visible light or radio waves -- could potentially be dangerous if highly concentrated into a narrow beam (that is the principle of lasers) of very high power.

5. What is the function of UV spectrophotometer?

UV / Vis spectrophotometer measures the absorbance of a light when it passes through a sample. The light absorbed is proportional to the quantity of a chemical in the sample.

Spectrophotometers